Yesterday spent on R code and writing…today the lab.
Due to possible (I don’t think I did, but can’t be 100% sure I didn’t) contamination between adapters P1.45 and P1.46, I remade stocks for these two adapters.
Made 10x dilutions of annealed primer stocks - 30:270 ul for a total of 300 ul so that I can put 2 ul in each of 96 rxns with plenty to spare.
Performed adapter ligation for both ApPopGen (JSG001) and ApAdaPt (JSG002) plates.
To experiment with gel excision, ran a large gel with 5 lanes of 1kb ladder.
Note - for real samples, run ladder on each side of sample and use both bands to circumscribe the region to excise.
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