30 September 2014
Phenol-Chloroform and DNANext post Previous post
Continued phenol-chloroform extraction using the Thermo Scientific Phenol/Chloroform Extraction and Ethanol Precipitation protocol.
At this point, very discouraged because I didn’t see a pellet in bottom of tube. Then remembered that because bottom of tube is rounded, Brian thinks I wouldn’t get a pellet but a thin uniform layer. A light brownish hue around bottom indicated this might be true…
Nanodrop: 145 ng/ul in 80 ul = 11.6 ug DNA!
Need fragment analyzer to determine if DNA fragment size is adequate.
Comments from co-authors
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