Tuesday notes

ApTranscriptome

Continuing working on analysis.

ApGXL

To check quality of RNA after RNAzol extraction only, diluted the 2ul aliquots from yesterday’s extractions 10x. Sent 2 ul for Bioanalyzer analysis (Pico range 200 pg/ul - 25 ng/ul).

If quality of DNA is adequate and concentration is not much lower, there should be enough RNA in the remaining 15-18 ul for DGE.

Also did a new round of RNA extraction through RNAzol only but different than yesterday:

all ethanol washes
elute in 102 ul H2O

RNAzol extraction
Sample	RNAzol ng/ul	RNAzol 260/280	BioAn ng/ul	BioAn RIN
20D CT	157.8	1.8	0	5.9
20D HS	26.0	1.6	0	1.2
24A CT	lost	-	-	-
24A HS	79.9	1.7	0	1

Given the greater elution volume, these values are on par with yesterday’s results.

Diluted these sample 10x and sent for Bioanalyzer (Pico range 200 pg/ul - 25 ng/ul) along with other samples.

UPDATE: 2014-01-15 added Bioanalyzer results. Terrible - shouln’t have diluted as any signal disappeared.

Other

Comments on undergrad research proposals by Grace and Nicole.