Continuing working on analysis.
To check quality of RNA after RNAzol extraction only, diluted the 2ul aliquots from yesterday’s extractions 10x. Sent 2 ul for Bioanalyzer analysis (Pico range 200 pg/ul - 25 ng/ul).
If quality of DNA is adequate and concentration is not much lower, there should be enough RNA in the remaining 15-18 ul for DGE.
Also did a new round of RNA extraction through RNAzol only but different than yesterday:
|Sample||RNAzol ng/ul||RNAzol 260/280||BioAn ng/ul||BioAn RIN|
Given the greater elution volume, these values are on par with yesterday’s results.
Diluted these sample 10x and sent for Bioanalyzer (Pico range 200 pg/ul - 25 ng/ul) along with other samples.
UPDATE: 2014-01-15 added Bioanalyzer results. Terrible - shouln’t have diluted as any signal disappeared.
Comments on undergrad research proposals by Grace and Nicole.
This work is licensed under a Creative Commons Attribution 4.0 International License.