17 January 2014
knitcitations, pander, and pandocNext post Previous post
continuing work on analysis…
Test extractions to see if I can figure out problems…
Set up 3 reps of each using ants from Andrew’s colonies in lab
For RNAzol, followed standard protocol. Took aliquots from 102 ul elution for Nanodrop and Bioanalyzer quantification, then proceed with Qiagen RNeasy with all samples.
For Qiagen only, homogenized ant with zirconium silicate beads in 350 ul Buffer RLT. Centrifuged. Transferred supernatant to new tube, added 350 ul 70% EtOH.
Sent samples for Bioanalyzer analysis:
where the first six samples were the RNAzol extractions, and the last three were the best (double) RNeasy extractions.
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