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Working on analysis, manuscript
Streptavidin bead cleaning of P1-P1 primer pairs
Do I need to remove DNA from beads prior to PCR? ddRAD protocol is vague on this…
According to Life Sciences FAQ, No:
Can the Streptavidin-Coupled Dynabeads® be used directly in a PCR reaction?
Yes, the beads can be used directly in PCR, as they do not inhibit enzymatic reaction. Slight PCR-inhibition has been seen when 75–100 µg of Dynabeads® M-280 Streptavidin was used in 50 µL PCR reaction, and a concentration over 100 µg per 50 µL reaction seems to inhibit PCR completely. Dynabeads® M-270 Streptavidin do not show any inhibitory effect on PCR at these concentrations.
KM practice talk
This work is licensed under a Creative Commons Attribution 4.0 International License.