uclust finished in 9 hours. Reduced assembly to 105,536 transcripts. Total reduction after CAP3 and uclust of only about 20,000 transcripts. Unlikely to be enough to resolve issue of unique transcripts from each colony for same gene. Next step is to
blastx against other ant transcriptomes.
Success! Yesterday’s trial of using Buffer ATL (from DNeasy kit) and Buffer G2 (from Genomic-tip kit) showed that the G2 buffer is not lysing cells.
Nanodrop results - average of 2-3 trials per sample
|G2 - aliquot 4||15.2||0.47|
|G2 - DNA||1.8||0.26|
|ATL - aliquot 4||26||4.4|
|ATL - DNA||22.2||1.63|
Aliquot 4 is the elution, prior to precipitating with isopropanol and ethanol.
The concentration of the G2 aliquot is suspect given the terrible quality (lower 260 than 280 peak). The wikipedia page on nucleic acid quantitation supports this showing a sample with 100% protein and 0% nucleic acid to have 260:280 at 0.57. The G2 DNA sample confirms this with DNA concentration near zero.
In contrast, the ATL aliquot has high concentration and a low 280 peak indicating little protein contamination. The ATL DNA has high concentration and good quality.
Testing out new text editors: Scribes has promise. Nice interface.
The “Markdown plugin” initially failed to work, but when running from the terminal I saw this error message:
Traceback (most recent call last): File "/home/jsg/.local/share/gedit/plugins/markdown-preview/__init__.py", line 86, in <module> with open(confFile, "wb") as confFile: IOError: [Errno 13] Permission denied: '/home/jsg/.config/gedit/gedit-markdown.ini'
Went to the specified file ‘/home/jsg/.config/gedit/gedit-markdown.ini’ and changed permissions
sudo chmod a+rwx gedit-markdown.ini
and ran fine after that!
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