Tuesday notes
Molecular Ecologist Q&A with Charles Goodnight posted.
ApTranscriptome
Working on analysis
ddRADseq
Bioanalyzer results - no DNA
Qubit - no DNA
Shit. Tomorrow begins trouble-shooting…
Reading
Murren, C.J., Maclean, H.J., Diamond, S.E., Steiner, U.K., Heskel, M.A., Handelsman, C.A., Ghalambor, C.K., Auld, J.R., Callahan, H.S., Pfennig, D.W., Relyea, R.A., Schlichting, Carl D. & Kingsolver, J. (2014). Evolutionary Change in Continuous Reaction Norms. The American Naturalist, 183, 453–467.
- metaanalysis of continuous reaction norms
- compare mean and shape of reaction norms among species and/or populations
- offset: overall difference in mean treat value across all environments
- slope: difference in overall slope
- curvature: average difference in curvature of the reaction norm
- wiggle: variability in shape not captured by previous three measures
- total of 3,079 contrast lines examined including six moderator variables
- differences in slope + curvature + wiggle greater than differences in offset
- offset greater for biotic interactions than abiotic factors
- greater differences in reaction norms between species than populations
- “results suggest that microevolutionary changes in curvature and higher-order shape (Wiggle) may be more evolutionary variable than simpler (first-order) aspects of the reaction norm such as slope”
- “two-environment studies may underestimate the extent of evolutionary change”
- divergence greater in novel environments; maybe indicative of ‘hidden’ plasticity
- Overall, interesting analysis with important results
- Many aspects useful for interpretation of thermal reactionome!
