FastQCFastQC Report
Tue 23 Jul 2013
A22-10-R2_val_2.fq

Summary

[OK] Basic Statistics

Measure Value
Filename A22-10-R2_val_2.fq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 18232041
Filtered Sequences 0
Sequence length 20-101
%GC 39

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTGGCT 92348 0.5065148767491253 No Hit
GTCCTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTG 84359 0.46269641451552246 No Hit
CTCATATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGC 61970 0.3398961202423799 No Hit
CACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTA 61823 0.33908984737364295 No Hit
CTGGCTTACACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAA 40384 0.2215001600753311 No Hit
ATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAAC 33940 0.18615579023763712 No Hit
GCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGA 33242 0.18232736532349836 No Hit
CAGCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTC 26283 0.14415829802050137 No Hit
CTTTGCACAGTCATTATACTGCGGCCATTTAAAAATCTCATGGGGCAGAA 26055 0.14290775234654202 No Hit
CTTCTCGTCCCATAATACTATTTAAGTTTTTTTACTTAAAAAATAATTTA 24301 0.1332873264161703 No Hit
ATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGCTACCT 23433 0.12852647709600917 No Hit
CAAATATTGAGCTCAACTGTTTATTAAAAACATAGCTTTTAGATTATAAT 23318 0.12789571940958228 No Hit
ATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGATGTTGAA 21746 0.11927353607859921 No Hit
TATTAATAAAAATGATTGCGACCTCGATGTTGAATTAAAATAAAAATTAG 20327 0.11149053471303624 No Hit
AATTAATAAAAGGAGTAATATTAATTTAAGGAATTAGGCAAATATTGAGC 19499 0.10694907937076271 No Hit
GCTACCTTTGCACAGTCATTATACTGCGGCCATTTAAAAATCTCATGGGG 19274 0.1057149882451449 No Hit
GTCTTCTCGTCCCATAATACTATTTAAGTTTTTTTACTTAAAAAATAATT 19051 0.10449186681842147 No Hit
CCTTTGCACAGTCATTATACTGCGGCCATTTAAAAATCTCATGGGGCAGA 18798 0.10310419990828236 No Hit
CAAGTTTTTAATTAAAAAACAATTGATTATGCTACCTTTGCACAGTCATT 18649 0.102286957340651 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GCGGC 1546930 2.8929508 5.3322625 70-74
CGGCC 1542510 2.8285966 7.283668 1
CGGCG 1337780 2.5018141 9.324031 1
CGCCG 1362255 2.498052 8.041611 1
CGATC 2751315 2.2188692 10.784728 4
CACCG 1820260 2.2052786 10.310156 1
GGTCG 1709590 2.128255 5.0023565 95-97
CGACG 1655575 2.0455317 6.0439463 1
CCTCG 1660890 1.9880071 6.036413 1
CTGGC 1613380 1.9694321 8.349347 1
CCGGC 1054670 1.9340143 7.003429 1
CGCGG 999475 1.8691419 5.8346643 1
CCGCG 1016605 1.864212 5.9919753 1
CCGAT 2220225 1.790558 8.984384 3
GCCGA 1419820 1.7542467 5.0835843 1
ACCGA 2033880 1.6602356 6.927492 2
GGCTT 2027570 1.647561 5.4039807 3
GTCGC 1321465 1.6130953 5.9177666 95-97
CAGCG 1269100 1.5680258 5.2654314 1
GACCT 1931345 1.5575831 6.2132654 95-97
GGGGC 795730 1.5176213 7.754477 95-97
CTTTC 2860840 1.4878731 7.497574 1
GATCT 2667105 1.4318341 6.958833 5
TCCTT 2680550 1.3941073 6.227093 2
CCTTT 2526865 1.3141785 6.336494 3
CTCGG 1037290 1.2662065 5.787603 1
CTAAA 3386810 1.21586 5.083024 8
TTTCG 2189940 1.1615338 6.502352 5
AGACC 1398920 1.1419243 5.246589 95-97
TTCGT 2097520 1.1125146 6.4719915 6
CGTAC 1333775 1.0756574 8.563856 5
GTCCT 1204150 0.95944244 8.515008 1
CCGGG 501415 0.9377082 5.0467434 1
GTACA 1705475 0.92672527 6.4330974 6
TCGTA 1704920 0.91528565 6.4002094 7
Produced by FastQC (version 0.10.1)