FastQCFastQC Report
Tue 23 Jul 2013
A22-21-R2_val_2.fq

Summary

[OK] Basic Statistics

Measure Value
Filename A22-21-R2_val_2.fq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14287639
Filtered Sequences 0
Sequence length 20-101
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GTCCTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTG 83135 0.5818666051122933 No Hit
CTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTGGCT 78766 0.5512877250048102 No Hit
CACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTA 57815 0.40465048144063553 No Hit
CTCATATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGC 45000 0.3149575657671642 No Hit
CTGGCTTACACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAA 35249 0.24670976079392823 No Hit
GCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGA 29102 0.2036865573101336 No Hit
CAGCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTC 26902 0.18828863187262782 No Hit
ATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAAC 25017 0.17509540939549215 No Hit
TATTAATAAAAATGATTGCGACCTCGATGTTGAATTAAAATAAAAATTAG 20029 0.14018411299445624 No Hit
GTTGCTTTTAGCCCTTGAGATCCGTGAATTTGTTAAATCCGTCAGTTGTT 19762 0.13831536477090442 No Hit
CAAATATTGAGCTCAACTGTTTATTAAAAACATAGCTTTTAGATTATAAT 19336 0.13533376648164192 No Hit
ATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGATGTTGAA 18873 0.13209320308274866 No Hit
TATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAA 16293 0.11403563597876458 No Hit
CCTCGATGTTGAATTAAAATAAAAATTAGATGTAGATGTCTAATATTTAG 16201 0.11339172273319616 No Hit
CCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTAAA 14816 0.10369802876458456 No Hit
ATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGCTACCT 14367 0.10055545216392996 No Hit
CGCAAAGCCTAAATTGGCTGATGCAAAGCCTAAATTGGCTGACGCAAAGC 14293 0.10003752194466839 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGCCG 1415125 3.1578689 4.4022326 25-29
CGGCC 1401975 3.0592272 6.4434967 1
CGGCG 1104565 2.4648502 8.669671 1
CGCCG 1122130 2.448582 8.039263 1
AGCCT 2313870 2.3273723 5.7013955 5
GCCGG 1022155 2.2809513 5.1429367 1
GGCTT 2207100 2.2443726 6.3057523 3
GGTCG 1465260 2.232105 5.701322 95-97
CACCG 1514510 2.2315075 11.239926 1
CGATC 2215200 2.2281265 11.203712 4
CCGGC 1020315 2.226413 7.4513493 1
CTGGC 1455785 2.1685503 8.641438 1
AAGCC 2040620 2.0762079 5.6653905 5
CCTCG 1397610 2.0357778 6.186933 1
TGGCT 1949130 1.982046 5.7007446 45-49
CCGAT 1949295 1.9606698 9.866384 3
GCCGA 1298110 1.9559845 5.8781095 1
CGACG 1236465 1.8630984 5.03646 1
CCGCG 842470 1.8383403 5.40082 1
CGCGG 820230 1.8303531 5.374421 1
GCAAA 2548525 1.7905236 5.5850415 2
CCTAA 2604970 1.7692301 5.094829 7
GACCT 1741775 1.7519389 6.7895007 95-97
AGGTC 1661255 1.7087989 5.035268 95-97
ACCGA 1645505 1.6742021 7.824359 2
CGCAA 1593570 1.6213613 5.3554955 1
CTAAA 3392195 1.5909075 6.737366 8
GGGGC 667540 1.5233663 7.684139 95-97
GATCT 2185335 1.5005331 7.4287825 5
GTCGC 1004260 1.4959545 6.4034653 95-97
TCCTT 2238255 1.4856882 7.5678606 2
CAGCG 984500 1.483439 5.65532 1
CTTTC 2122950 1.409152 7.6616926 1
CGAAC 1375155 1.3991373 5.3830347 90-94
CAAAT 2795675 1.3111451 5.122001 9
CCTTT 1944665 1.2908117 7.3323026 3
AGACC 1257940 1.2798781 5.8544984 95-97
CTCGG 789545 1.176113 5.031457 1
TTCGT 1708195 1.1595336 7.9884715 6
CAGAC 1135295 1.1550943 5.3758826 95-97
TTTCG 1619755 1.0994998 7.761055 5
CGTAC 1067410 1.0736388 10.2454195 8
TCGTA 1320870 0.906959 7.7733006 7
GTCCT 897595 0.89253575 9.987317 1
GTACA 1171365 0.81358266 7.048585 9
TACAA 1690815 0.79297626 5.1483407 7
Produced by FastQC (version 0.10.1)