FastQCFastQC Report
Tue 23 Jul 2013
A22-31-R1_val_1.fq

Summary

[OK] Basic Statistics

Measure Value
Filename A22-31-R1_val_1.fq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14239058
Filtered Sequences 0
Sequence length 20-101
%GC 39

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTGGCT 65669 0.4611892163091126 No Hit
GTCCTTTCGTACAAATAATTTAATACTAATTATAGATAGAAACCGATCTG 56929 0.3998087513935262 No Hit
CTCATATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGC 47221 0.33163008395639654 No Hit
CACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAACAGACCTA 42654 0.29955633301023143 No Hit
ATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAAC 29689 0.20850396142778546 No Hit
GCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGA 29516 0.2072889934151543 No Hit
CTGGCTTACACCGATCTAAACTCAAATCATGTAAAATTTTAAAGGTCGAA 28932 0.20318759850546292 No Hit
CAGCGTAATATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTC 21248 0.1492233545224691 No Hit
ATTTTTAGAAAGATCATATTAATAAAAATGATTGCGACCTCGATGTTGAA 20300 0.14256561073070986 No Hit
TATTAATAAAAATGATTGCGACCTCGATGTTGAATTAAAATAAAAATTAG 19545 0.13726329368136572 No Hit
CAAATATTGAGCTCAACTGTTTATTAAAAACATAGCTTTTAGATTATAAT 19294 0.13550053662257713 No Hit
ATTTTCTTTCATCCAAGTTTTTAATTAAAAAACAATTGATTATGCTACCT 18050 0.12676400363001541 No Hit
CTTTGCACAGTCATTATACTGCGGCCATTTAAAAATCTCATGGGGCAGAA 17698 0.12429192998581788 No Hit
AATTAATAAAAGGAGTAATATTAATTTAAGGAATTAGGCAAATATTGAGC 16023 0.11252851136641202 No Hit
CTTCTCGTCCCATAATACTATTTAAGTTTTTTTACTTAAAAAATAATTTA 15843 0.11126438279835647 No Hit
TATTAATTTAAGGAATTAGGCAAATATTGAGCTCAACTGTTTATTAAAAA 15460 0.10857459812299382 No Hit
CAAGTTTTTAATTAAAAAACAATTGATTATGCTACCTTTGCACAGTCATT 14982 0.1052176344811574 No Hit
GCTACCTTTGCACAGTCATTATACTGCGGCCATTTAAAAATCTCATGGGG 14486 0.10173425798251541 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CGGCC 1273245 3.180394 7.6219873 1
GCGGC 1242870 3.128366 6.3483005 70-74
GGCCG 1182000 2.9751532 5.653399 9
CGGCG 1019600 2.5663843 8.30305 1
CGCCG 999185 2.495829 7.355542 1
CACCG 1447190 2.3460312 10.820562 1
CGATC 2150610 2.273427 10.732451 4
GGTCG 1383915 2.271498 5.2027445 95-97
CCTCG 1403780 2.269113 6.1683335 1
CTGGC 1304285 2.124479 9.065635 1
CGACG 1270285 2.0750682 5.787998 1
CCGGC 819605 2.0472624 6.9228625 1
GCCGG 810275 2.0395029 5.0775843 1
CGCGG 761890 1.9177153 5.7939587 1
CCGCG 759105 1.8961416 5.7049956 1
CCGAT 1746285 1.8460116 8.965292 3
GACCT 1692220 1.7888594 7.0961924 95-97
ACCGA 1633105 1.731349 7.151468 2
CAGCG 1047785 1.7116041 6.193551 1
GGGGC 665430 1.6877851 8.966013 95-97
GGCTT 1527010 1.6219332 5.787745 3
GTCGC 987240 1.6080617 5.9346614 95-97
TGGCT 1428810 1.5176287 5.0428543 45-49
CTTTC 2223240 1.5165007 8.144665 1
GATCT 2122690 1.4632447 6.9576573 5
TCCTT 2082130 1.4202477 6.3827543 2
CCTTT 2020695 1.3783422 6.496526 3
CTCGG 788165 1.2837993 5.4538345 1
ACACC 1136265 1.1954385 5.0710263 8
CAGAC 1115315 1.1824099 5.2526455 95-97
AGACC 1110745 1.1775651 5.543226 95-97
TTTCG 1649155 1.1335502 6.6471434 2
CGTAC 1048135 1.1079919 9.441948 5
TTCGT 1608515 1.1056162 6.567475 6
CTCAT 1606730 1.0991333 5.4446893 1
GTCCT 912245 0.96156716 8.739699 1
CCGGG 377165 0.9493431 5.006303 1
GTACA 1323515 0.9149776 6.8031416 6
TCGTA 1316460 0.90748215 6.4331217 7
TACAA 1810760 0.8124223 5.057189 7
Produced by FastQC (version 0.10.1)