Regulation of gene expression

John Stanton-Geddes
18 November 2013

Overview

Part 1)

Regulation of gene expression


Part 2)

How do bacteria do what they do?

Types of gene expression

  • Constitutive


  • Inducible


  • Repressible


Gene expression 1

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Is tryptophan being synthesized by E. coli?

Gene expression 2

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Is tryptophan being synthesized by E. coli?

Gene expression 3

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Is tryptophan being synthesized by E. coli?

Gene expression 4

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Is tryptophan being synthesized by E. coli?

Gene expression 5

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Is tryptophan being synthesized by E. coli?

Gene expression 6

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Is lactose being metabolized by E. coli?

Gene expression 7

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Is lactose being metabolized by E. coli?

Gene expression 8

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Is lactose being metabolized by E. coli?

Gene expression 9

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Is lactose being metabolized by E. coli?

Gene expression 10

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Is lactose being metabolized by E. coli?

Positive gene regulation

cAMP

Bacteria communication

How do bacteria do what they do?

biofilm

http://en.wikipedia.org/wiki/Biofilm

Bacteria communication

How do bacteria do what they do?

John Snow map

Johnson, S.B (2006) “The Ghost Map” Riverhead Trade.

Bacteria communication

Quorum sensing


Work on case study in groups of 3 - 4

Manipulating bacteria

Eukaryotic gene expression

nucleus

cytoplasm