Summary of sample treatment for transcriptomic studies

First author	Year	Journal	System	Sample treatment	Sequencing
Yek	2013	Molecular Ecology	leaf-cutting ants	workers of same approximate age from field-collected lab colonies, exposed to fungus	RNAseq
Whitehead	2013	Molecular Ecology	killifish	adults kept in lab conditions for 6 months prior to experiments. treated “at salinities that were physiologically challenging for each species”	microarray
Barshis	2013	PNAS	coral	samples collected and exposed to control and heated temps in outdoor flow-through aquaria for 72 h	RNAseq
Kvist	2013	Molecular Ecology	Glanville fritillary butterfly	larvae from F2 families in lab	microarray
Teets	2012	PNAS	antarctic midge	field-collected larvae	RNAseq
Runcie	2012	Molecular Ecology	sea urchin	full- and half-sib families generated from field-collected adults	RNAseq
Heinrich	2012	PLoS ONE	kelp	sporophytes grown from field-collected gametophytes	microarray
Meyer	2011	Molecular Ecology	coral	full-sib larval families by cross-fertilizing games from three different colonies	RNAseq
Ometto	2011	Molecular Biology and Evolution	fire ants, two species	individuals sampled from lab colonies two weeks after field collection	microarray
Siebert	2011	PLoS ONE	cnidaria	field-collected samples,	RNAseq
Wolf	2010	Molecular Ecology	crows, two species	field-collected	microarray
Holliday	2008	New Phytologist	Sitka spruce	4 yr-old spruce saplings grown in common garden from seed collected from natural populations	microarray
Whitehead	2006	PNAS	killifish	field collected and acclimated in lab 2 months before experiments	microarray