15 April 2014
ddRADseq and restriction enzymesNext post Previous post
Heat shocked ‘blue’ ants.
Started trial ddRADseq library prep using 12 expendable Aphaenogaster samples that Nicole extracted last week - 3 ants from each colony.
Measured concentration using Qubit.
|Sample||Colony||ng/mL||Total DNA (ng)|
Total DNA =
Qubit sample ng/mL * 100 * (1/1000 ul) * 40 ul
Combined Total DNA = 8 ug
For ‘real’ library would pool 24 - 48 samples, here only using 6 so used all DNA from each sample to ensure large volume of DNA in pooled sample for size selection and purification steps.
50 ul rxn volume following NEB protocol
3 hr digestion at 37°C.
Included “C” Control sample of dH2O only.
Sent 2ul aliquot of each sample for Bioanalyer analysis of cutting efficiency.
Started PLOS Biology review
Cool video of Rob Dunn talking about making important scientific discoveries
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