Heat shocked ‘blue’ ants.
Started trial ddRADseq library prep using 12 expendable Aphaenogaster samples that Nicole extracted last week - 3 ants from each colony.
Measured concentration using Qubit.
|Sample||Colony||ng/mL||Total DNA (ng)|
Total DNA =
Qubit sample ng/mL * 100 * (1/1000 ul) * 40 ul
Combined Total DNA = 8 ug
For ‘real’ library would pool 24 - 48 samples, here only using 6 so used all DNA from each sample to ensure large volume of DNA in pooled sample for size selection and purification steps.
50 ul rxn volume following NEB protocol
3 hr digestion at 37°C.
Included “C” Control sample of dH2O only.
Sent 2ul aliquot of each sample for Bioanalyer analysis of cutting efficiency.
Started PLOS Biology review
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