22 April 2014


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Tuesday notes


Bioanalyzer results

Discouraging - no DNA in the pooled samples that should have DNA. There is low-level DNA in the ligated samples - actually less than after the double digestion.

Potential problems:

  • low recovery from AMPure purification during pooling
  • low recovery from gel purification

Though I couldn’t find anything specific about percent DNA recovery following AMPure bead purification on Beckman Coulter’s website, this awesome lab page gave results showing about 70% recovery with 1.4 - 1.6 bead ratio. Seems okay.

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