11 September 2014
RADseq, Illumina, and fastqcNext post Previous post
Two libraries were sequenced on Illumina HiSeq 1500 in RapidRun mode at VCC.
Lane 1 - RAD4: repeat of Pogonomyrmex RAD-seq
--with-failed-reads) conversion due to low complexity in restriction site region
Lane 2 - ddRAD1: first 48 samples of Aphaenogaster double-digest RADseq protocol 
 Peterson, B.K., Weber, J.N., Kay, E.H., Fisher, H.S., and Hoekstra, H.E. (2012). Double Digest RADseq: An Inexpensive Method for De Novo SNP Discovery and Genotyping in Model and Non-Model Species. PLoS ONE 7, e37135.
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