28 January 2014
RSEM and double digestNext post Previous post
blastnefficiently for Chagas RAD-tags…
Heat-shocked one group (blue) of ants for 1 hour at 42C. Red group kept in tubes at room temp.
Mahesh sent RSEM output for the “Trinity_cap3_uclust.fasta” assembly.
Within both data sets, expression values are highly correlated between colonies,
but between data sets, there is NO correlation…how can this be???
UPDATE - transcripts were ordered differently. when
mergeed, correlations > 0.94 between RSEM and sailfish expression values.
Correlations between colonies at each temperature level are also high, > 0.9 for all temperatures except 7.5 C
NlaIII - MluCl double digest for 6 DNA samples.
Made a worksheet to calculate reaction volumes available here.
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