Grace observed colony for about 40 minutes - observed 35 blue (heat-shocked) and only 7 red (control) ants foraging!
Confirmed that RSEM and sailfish expression values are highly correlated, and that expression values are correlated between temperature levels for both colonies.
Re-run of script identifies unique set of responsive transcripts. Must have been bug in the sailfish step that caused duplication problems. Now fixed!
Updated and merged branch to use
ddply instead of
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