Meet with Nicole to do AmPure Bead purification of double digest…but PCR plates and tubes don’t fit the magnetic plate. Had to order 300 ul round bottom PCR plates.
Debugged analysis and repeatable results.
50% speed improvement using
ddply over for loop to identify significant transcripts.
FDR correction is too conservative. For A22, while 11,846 transcripts have uncorrected P<0.05, only 4 are significant at 5% FDR, and none for Ar.
Split the analysis into ‘low’ and ‘high’ temps as discussed yesterday, and the result is no different.
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