This work is licensed under a Creative Commons Attribution 4.0 International License.
A third attempt at the ddRADseq protocol.
DNA extraction
Completed DNA extraction that I started yesterday.
Eluted in 25ul Buffer AE. For samples 1-11, eluted twice with same buffer AE to see if this would increase yield. Reserved 2ul for Qubit.
Double digest
50 ul rxn volume following NEB protocol
| Reagent | Volume (ul) |
|---|---|
| NlaIII | 1 |
| MluCl | 1 |
| DNA | 20 |
| CutSmart Buffer | 5 |
| H2O | 23 |
3 hr digestion at 37°C.
Stored samples in fridge.
This work is licensed under a Creative Commons Attribution 4.0 International License.