06 January 2014

blast and RNA extraction

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Monday Jan 6 notes

This is awesome - print a cloth scientific poster and stuff it in your backpack!


Mahesh @ VGN also working on transcriptome. They have blast2GO databases locally and may be able to do mapping and annotation.


Prep for RNA extractions!

NuGen Ovation requires only 10ng RNA per sample, so can do with single ant.

Other things

Running blast for chagas RADseq hits against NCBI nt database for Sara. Test run with only 100 sequences

Had to download nt database first

perl update_blastdb.pl nt

for i in 00 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16
    tar -xvf nt.$i.tar

And blast text

blastx -query test100.fasta -db nt

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