This is awesome - print a cloth scientific poster and stuff it in your backpack!
Mahesh @ VGN also working on transcriptome. They have blast2GO databases locally and may be able to do mapping and annotation.
Prep for RNA extractions!
NuGen Ovation requires only 10ng RNA per sample, so can do with single ant.
Running blast for chagas RADseq hits against NCBI nt database for Sara. Test run with only 100 sequences
Had to download nt database first
perl update_blastdb.pl nt for i in 00 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 do tar -xvf nt.$i.tar done
And blast text
blastx -query test100.fasta -db nt
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