Based on recommendation, used FastAnnotator for annotation - GREAT web resource!
Results for AphVT assembly.
Meeting with Mahesh
Discussed transcriptome assembly and analysis.
Advocates using full (not digitally-normalized) assembly with all reads from both colonies
RNA extraction with 3 samples from ApTranscriptome. Trial run and will use these to evaluate DGE against full RNAseq.
Took about 5 hours for complete protocol.
Total yield 140-600 ng per sample. Well above minimum of 10 ng for DGE kit.
Comments to Yun on Medicago GWAS manuscript
blastn job for Chagas on mason cluster
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