Bioanalyzer HS results in.
Each sample has a tiny peak at 68-69 bp. Are these the adapters? What happened to DNA???
Meeting with Nick
Concerned that simple
lm maybe too liberal in detecting patterns. After some reading, decided that I should use robust regression for analysis of gene expression data. No default F statistic to assess overall model fit, but this page explains that fitting a full model and a minimal model with no covariates
Y ~ 1 and then doing
anova(full.model, minimal.model) is equivalent to an F test. I confirmed this for the simple
Error message where model doesn’t fit - need to continue
ddply even with error. Using
try as suggested here.
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Botkin, D., Saxe, H., Araujo, M.B., Betts, R., Bradshaw, R.H.W., Cedhagen, T., Chesson, P., Dawson, T.P., Etterson, J.R., Faith, D.P., Ferrier, S., Guisan, A., Hansen, A., Hilbert, D.W., Loehle, C., Margules, C., New, M., Sobel, M.J. & Stockwell, D.R.B. (2007). Forecasting the Effects of Global Warming on Biodiversity. BioScience, 57, 227–236.
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