28 June 2013
Aphaenogaster and MedicagoNext post Previous post
Finished edits to ‘Genomic estimation of heritability’ manuscript and sent to co-authors.
Had planned to collect colonies at Red Rocks but afternoon thunderstorms got in the way…
Drove to Harvard Forest. Arrived about 1pm and quickly begin searching for Aphaenogaster colonies with goal of collecting 50.
Aphaenogaster very common! Found 25 colonies in 4 hours of searching with help from Monica Davis.
Collecting at Harvard Forest - 25 additional colonies for a total of 50!
Only found 1 more queen, though was working quickly to get quantity of colonies.
Plans for sampling local sites with Kerri and Skyler.
Early morning went out collecting for common garden colonies at Red Rock. Difficult to find colonies – too wet. Found 2 small colonies in about an hour before going to East Woods (Stonehedge access). Much better! Found 2 colonies within 15 minutes, and 9 colonies in 90 minutes. In the last colony, finally found a queen!
Finished 3 colonies in about 2 hours
Finished preparing composite colonies.
** Thought - should get CTmax/min for each of the host colonies as well. **
Joel, Kerri and Skyler helped with transfer of larvae to composite colonies.
A damn tricky issue. Mentioned to Sara and she commented that one strategy (her PhD advisor?) was to just label ALL samples collected sequentially and keep one big log file. As in
JSG0001 | Chamaecrista | Date | Location | Lat | Long | Description JSG0002 | Chamaecrista | Date | Location | Lat | Long | Description JSG0102 | Aphaenogaster | Date | Location | Lat | Long Description
For lack of better ideas, I think I’ll adopt this. But how to label samples in experiment? Seems I could also label all experiments sequentially. In fact, I actually started to do this with Chamaecrista
|ID||Short Name||Year Started||Description|
|JSG-E01||CfComp||2007||Effect of competition of Chamae fitness|
|JSG-E02||CfHab||2008||Effect of soil type and range location on Chamae fitness|
|JSG-E03||CfRL2||2009||Chamae range limit experiment|
|JSG-E04||CfRhiz||2009||Chamaecrista - rhizobia range limit experiment|
|JSG-E05||CfPopGen||2009||Chamaecrista population genetics|
|JSG-E06||CfBe||2010||Geographic variation in Chamaecrista-Bradyrhizobium symbiosis|
|JSG-E09||MtGCTA||2011||Medicago genomic estimation of heritability|
|JSG-E10||MxE||2012||Medicago Experimental Evolution|
|JSG-E12||GxL||2013||Aphaenogaster Gene eXpression by Latitude|
|JSG-E13||AphAdaPt||2013||Aphaenogaster Adaptive Potential|
Label composite colonies
JSG-E13-01, JSG-E13-02, etc
Maintain csv file with information for each colony in /project/climate-cascade/APt/planning directory
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